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Old 07-30-2012, 01:00 AM   #1
darren.obbard
Junior Member
 
Location: Edinburgh, UK

Join Date: Jan 2012
Posts: 5
Default small-RNA library prep from synthetic siRNAs

Hi all!

Does anyone know of a study (published or ongoing) that sequences (by illumina) small RNAs from a commercial source?

For example, has anyone used commercial siRNAs to perform a knock-down, and then sequenced the small-RNAs from that knock-down?

I am particularly interested in any issue regarding end-modification, and in the rate at which the artificial siRNAs are recovered relative to naturally occuring siRNAs.

Thanks!

Darren
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knock-down, mirna library, sirna, truseq

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