Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Nextera mate pair kit substitutions - Covaris and Dynabeads pjuneja Sample Prep / Library Generation 4 07-18-2014 03:16 AM
Assembly of nextera mate pair libraries agseq Bioinformatics 1 03-18-2014 02:39 AM
Nextera Mate Pair: H.pylori vs. C.crescentus bmc Sample Prep / Library Generation 0 10-31-2013 12:40 PM
Use of illumina mate pair libraries for scaffolding glacerda Bioinformatics 2 07-13-2011 07:35 AM
Sequencing Illumina mate pair libraries beyond 36bp PNG Sample Prep / Library Generation 2 08-24-2010 11:54 AM

Thread Tools
Old 04-29-2014, 07:04 PM   #1
Location: New York

Join Date: Dec 2011
Posts: 20
Default Illumina Nextera Mate Pair libraries.

I am curious about the directions for the Zymo Genomic DNA Clean & Concentrator recommended by the Illumina kit. It says to add 2 volumes of the DNA Binding Buffer to the tagmentation reaction and "mix thoroughly." What sort of mixing? flicking the tube? Pipetting? I am assuming that flicking the tubes is the safest thing to do here since I don't want to shear the DNA at this point.

Many thanks to anyone with experience and advice.

- Gina
Gina_P is offline   Reply With Quote
Old 04-30-2014, 05:35 AM   #2

Posts: n/a

Hi Gina,

Yes, you can try to flick the tube 5 times or so. Generally, flicking is not advisable when handling long DNA fragments, but they advise it throughout the whole Nextera MP prep.

If you really want to be sure your DNA gets minimal shearing/fragmenting while mixing - mix the liquid by pipetting with wide orifice tips. IMHO, this is the safest way.
  Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:14 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO