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Old 05-17-2016, 07:53 PM   #1
nareshmvr
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Location: India

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Default RNA-Seq vs qRT-PCR

I am attaching a figure based on assessing qRT-PCR vs Dual-RNASeq for selected Genome1 genes (black circles) and Genome2 genes (red circles).

I have analysed the correlation by Spearman Rank Order Correlation analysis:

Correlation Coefficient: 0.727
P Value: 0.00946
Number of Samples: 11

So, overall there is a significant correlation between the two datasets.

BUT, there is something slightly strange going on here. For Genome1, RNASeq seems to underestimate the changes in gene expression compared with qRT-PCR. Genome-2, it is the opposite - RNASeq overestimates gene regulation.
I have absolutely no idea why RNASeq should specifically underestimate Genome-1 gene regulation. please give your valuable suggestions in interprating for this qRT-PCR Validations
Attached Files
File Type: pdf Regression qRT-PCR vs RNASeq Sg Vp.pdf (103.3 KB, 20 views)

Last edited by nareshmvr; 05-17-2016 at 07:56 PM.
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Old 05-19-2016, 01:39 AM   #2
nareshmvr
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Dear All,

Am still wondering about this issue , I think my question is not unclear , Please suggest me if there are any possible ways to debug my issue.
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