SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
AMPure XP vs SPRI beads Tali7 Sample Prep / Library Generation 19 07-12-2017 11:13 AM
Why aren't my SPRI beads doing a size-selection? jwfoley Sample Prep / Library Generation 14 11-11-2016 07:58 AM
Illumina Sample Purification Beads vs AMPure XP Beads JER Illumina/Solexa 2 03-29-2015 01:36 AM
Homemade AMPURE beads size selection issue Todd McLay Sample Prep / Library Generation 0 06-19-2014 07:23 PM
Good Beads / Best Beads paolo.kunder SOLiD 2 03-27-2013 02:12 PM

Reply
 
Thread Tools
Old 05-30-2016, 06:12 AM   #1
JBKri
Member
 
Location: Greece

Join Date: Jan 2014
Posts: 75
Default Homemade SPRI beads- hydrophobic or hydrophilic beads?

We want to make our own Ampure beads for DNA cleanup and size selection.
We think we need the Sera-Mag Carboxylate-Modified SpeedBeads, but they come in two types; hydrophilic and hydrophobic. We are thinking the hydrophilic is what we need, but here they link to the hydrophobic variety.
JBKri is offline   Reply With Quote
Old 06-02-2016, 06:39 AM   #2
Don Stephano
Junior Member
 
Location: Germany

Join Date: Apr 2015
Posts: 2
Default

Hi,

I'm using Fisher #09-981-123 (GE Life Science 65152105050250) according to the protocol posted by Ethan-omics: https://ethanomics.wordpress.com/201...pure-xp-beads/

No problems so far, the selfmade beads are working perfectly.
Don Stephano is offline   Reply With Quote
Old 06-03-2016, 12:01 PM   #3
jwfoley
Senior Member
 
Location: Stanford

Join Date: Jun 2009
Posts: 181
Default

Quote:
Originally Posted by Don Stephano View Post
Hi,

I'm using Fisher #09-981-123 (GE Life Science 65152105050250) according to the protocol posted by Ethan-omics
Those are the same ones in the protocol JBKri is looking at, since we just copied the purchasing information when we improved on the other aspects of Faircloth & Glenn's protocol.

JBKri, why do you think they should be hydrophilic beads? Or, why do you think the carboxyl coating is hydrophobic?

Last edited by jwfoley; 06-03-2016 at 12:06 PM.
jwfoley is offline   Reply With Quote
Old 06-04-2016, 02:20 AM   #4
JBKri
Member
 
Location: Greece

Join Date: Jan 2014
Posts: 75
Default

Quote:
Originally Posted by jwfoley View Post
Those are the same ones in the protocol JBKri is looking at, since we just copied the purchasing information when we improved on the other aspects of Faircloth & Glenn's protocol.

JBKri, why do you think they should be hydrophilic beads? Or, why do you think the carboxyl coating is hydrophobic?
Thank you both! A colleague uses the homemade beads, and at one point there was some confusion about the beads; we were mistakenly given a product code for hydrophilic beads.
JBKri is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 10:52 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO