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Old 07-22-2017, 05:37 AM   #1
Junior Member
Location: Taiwan

Join Date: Oct 2013
Posts: 1
Unhappy 12 mer for Miseq custom sequencing primer

Does anyone had read the article below?

Treseder et al., 2016

According to the Material and Methods (attachment), they wanted to use stagger strategy from (Tremblay et al., 2015) but the PAD region has only 12bp, I used custom Index primer and custom R2 sequencing primer based on PAD sequence for a Miseq sequencing run but got a fail run when index run had started. I think the 12mer Index primer might be the problem. Here's me custom primer

Index sequencing primer
Read 2 sequencing primer

Can anyone help me for this issue?
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File Type: png 未命名-2.png (42.9 KB, 6 views)
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