SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Small RNA-seq with UDIs for Illumina® NovaSeq®, MiSeq®, & HiSeq® 2000/2500 sequencers PerkinElmer AG Vendor Forum 0 03-20-2019 11:21 AM
Base composition vs cycle, MiSeq vs HiSeq X JBKri Illumina/Solexa 3 07-03-2018 01:08 PM
In BASH splitting into substring based on pattern(or) last occurrence of a digit (**) AnushaC Bioinformatics 3 03-28-2016 01:21 PM
Hiseq imaging problems - dark edge MLog Illumina/Solexa 1 02-18-2013 05:24 AM

Reply
 
Thread Tools
Old 04-18-2019, 03:08 AM   #1
NGS_Man
Junior Member
 
Location: Germany

Join Date: Apr 2019
Posts: 2
Default Hello and dark digit cycle on HiSeq and NovaSeq

Hi at all,

im new in the forum and i want say just hi. Hope we have a nice time.

Anyway, my question is about the dark digit cycles on HiSeq and NovaSeq.

First, what does it mean and what is happening in this cycles??
How many cycles are dark digits and so on...


Thanks and best regards,

NGS_Man
NGS_Man is offline   Reply With Quote
Old 04-18-2019, 03:29 AM   #2
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 6,941
Default

Look on page 5 of this document from Illumina. I believe you are referring to "chemistry-only" cycles described there.

There is also a past thread on this: http://seqanswers.com/forums/showthread.php?t=29852
GenoMax is offline   Reply With Quote
Old 04-18-2019, 04:04 AM   #3
NGS_Man
Junior Member
 
Location: Germany

Join Date: Apr 2019
Posts: 2
Default

Thanks a lot, genomax
NGS_Man is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:39 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO