Sorry to bother everyone, but I am having problems with my conversion.
So, my sample has 0.05 ng/ul
This is the same as 0.05 kg/L
0.05 kg/L x 1 mol / 330 kg [based on 1 kb length ssDNA]
= 0.000151 mol/L
I have 100 ul of this so:
0.000151 mol/L x 0.0001 L = 1.51 x 10^-8 M = 15.1 nM
When I take 24 ul of this pooled sample, and add it to 576 ul of buffer, does that mean that I have...
15.1nM x 0.000024 L = 0.0003624 nmoles
0.0003624 nmoles / 0.0006 L (600 ul) = 2.17 x 10 ^ -7 nM
so that is putting me at 0.000217 pM? So I need to concentrate my sample more than the kit says?
I want to cluster at around 8 pM. So do I go: x/0.006 = 0.008nM (desired concentration
So, my sample has 0.05 ng/ul
This is the same as 0.05 kg/L
0.05 kg/L x 1 mol / 330 kg [based on 1 kb length ssDNA]
= 0.000151 mol/L
I have 100 ul of this so:
0.000151 mol/L x 0.0001 L = 1.51 x 10^-8 M = 15.1 nM
When I take 24 ul of this pooled sample, and add it to 576 ul of buffer, does that mean that I have...
15.1nM x 0.000024 L = 0.0003624 nmoles
0.0003624 nmoles / 0.0006 L (600 ul) = 2.17 x 10 ^ -7 nM
so that is putting me at 0.000217 pM? So I need to concentrate my sample more than the kit says?
I want to cluster at around 8 pM. So do I go: x/0.006 = 0.008nM (desired concentration
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