Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
quuestions virus genotyping application Juergen F Illumina/Solexa 0 02-14-2012 06:22 PM
Sample/library prep of DNA and RNA in a metagenomic sample chrisaw01 Metagenomics 1 05-05-2011 01:59 PM
Group Leader - Next Generation Sequencing Sample Prep New England Biolabs Industry Jobs! 0 04-04-2011 08:04 AM
AIR™ DNA Sample Prep Kits Now Available for Illumina-Compatible Sequencing Libraries Bioo Scientific Vendor Forum 0 10-05-2010 10:12 AM
questions about Illumina mRNA sample prep ik76 Illumina/Solexa 2 01-19-2010 03:38 AM

Thread Tools
Old 02-21-2012, 09:09 AM   #1
Juergen F
Junior Member
Location: California + Germany

Join Date: Jan 2012
Posts: 3
Default Questions sample prep for virus genotyping sequencing



0 – How do I guarantee a certain # of redundant reads per virus target gene area to be utilized later in assembly?

1 - I assume I would need a virus extraction method (Qiagen, Roche) performed upfront prior starting e.g. an Illumina multiplexing workflow with library prep?

2 - Which sample prep methods do match the input bp size requirements best (... output bp size of sample preps)?

3 - What input virus concentration (copies/ IU) do I need to start e.g. the Illumina multiplexing workflow?

Thanks & Greetings
Juergen F is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 02:58 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO