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Old 04-21-2017, 04:41 AM   #1
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Location: Oxford

Join Date: May 2016
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Default Cutadapt trimming issues

Hi, perhaps someone could advise me on the following issue.

I was trimming my paired-end reads with cut adapt using the following command:
cutadapt  -a CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT --minimum-length 45 -o ~/Virus_S5_trimmed_min45_R1.fastq.gz -p ~/Virus_S5_trimmed_min45_R2.fastq.gz Virus_S5_R1.fastq.gz Virus_S5_R2.fastq.gz
I the ran FastQC for the trimmed reads and in the overrepresented sequences section for the read 2 I have sequences all of which contain ATTGATGGTGCCTACAG in their 5' followed by different gene sequences.

This sequence is from one of the adaptors (I underlined it in the code), but I don't understand why does the cutadapt not trimm it off.

Any help would be appreciated.
Bernadeta is offline   Reply With Quote

cutadapt, fastqc, trimming reads

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