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Old 06-17-2008, 05:47 AM   #1
baohua100
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Location: Canada

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Question Questions about solexa quality score

reads.fq file:

@4:1:518:715
GATACCATAAAAGCTGGATCCTTCTTCAAGCATAA
+4:1:518:715
hhhhhhhhhhhhhhhdhhhhhhhhhhhdRehdhhP

1. How to change character (like 'e' or 'h') to quality score?

2. What's the meaning of this score? How to compute this score ( formula )?
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Old 06-17-2008, 08:09 AM   #2
myrna
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Thumbs up Converting single-character scores to their numeric values

Heng Li has provided a nice summary with examples (in Perl) here:

http://maq.sourceforge.net/fastq.shtml
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