SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Balance many PCR products for pooling Marius Sample Prep / Library Generation 4 05-02-2014 05:21 PM
Sequencing Short PCR Products Dynamac Sanger/Dye Terminator 4 07-23-2012 09:27 AM
Sequencing PCR products windhorse8 Introductions 0 05-27-2011 11:27 AM
Ligate pcr products for sequencing? seqgirl123 Illumina/Solexa 2 01-23-2011 11:55 AM
Sequencing for PCR products of different sizes deepakpatilp Illumina/Solexa 0 10-25-2010 07:15 AM

Reply
 
Thread Tools
Old 02-05-2015, 03:34 AM   #1
SylvainL
Senior Member
 
Location: Geneva

Join Date: Feb 2012
Posts: 175
Default Which kit for PCR products

Hi all,

I was wondering which Illumina kit you use to make libraries from PCR products (without phosphate on the 5') ?

Thanks in adavance,

s.
SylvainL is offline   Reply With Quote
Old 02-05-2015, 08:42 AM   #2
rnaeye
Member
 
Location: East Cost

Join Date: May 2011
Posts: 79
Default

Most likely, it does not matter what kit, as long as kit has an end repair step. I have been using NEB's kits for complex and PCR DNA without any problem.
rnaeye is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:47 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO