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Old 03-07-2015, 09:13 AM   #1
Snake75
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Default Directional/non-directional libraries vs paired-end/single sequencing

Hi,

Maybe this question will sound strange or silly but I don't see the relationship between a directional or a non directional library and a paired-end or single end sequencing.

For example, for paired-end sequencing, should the library be absolutely a directional library ?

I don't see if the type of library has an influence on the type of sequencing you want to perform.

Thanks

Snake75
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Old 03-07-2015, 09:24 AM   #2
fanli
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You can mix and match directionality and single/paired-end libraries. Although at this point I'm not sure that there's much of a reason not to do directional libraries.
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Old 03-07-2015, 11:46 AM   #3
Snake75
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Thanks fanli.

Indeed, I suppose that paired end sequencing on non-directional libraries must be difficult to interpret (?).
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