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Old 11-06-2015, 10:54 AM   #1
Ancestro
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Location: Mexico

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Default Ampure XP DNA rebinding if accidentaly added RSB

Hi to all!

I was preparing a library, but by accident (my mind was on other place i think) i added the RSB (Resuspension Buffer from Truseq RNa library prep kit) after the EtOH 80% wash, but the ethanol was not completely dried...

Did I messed up this library? Cause the RSB elutes the DNA from the beads. Is there a way to recover the DNA, or just Im a little paranoic right now and my DNA will still be fine?
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Old 11-06-2015, 05:19 PM   #2
nucacidhunter
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If you have collected RSB from beads you can dilute the eluate to reduce Ethanol concentration and do a column or another bead clean up.
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