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Old 12-14-2015, 09:13 AM   #1
SDPA_Pet
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Location: US

Join Date: Apr 2013
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Default Upload Illumina raw seqs to NCBI SRA

Hi, I am switching from 454 sequencing to Illumina. Now, I am going to upload my Illumina amplicon seqs to NCBI SRA.

What format should I use? For 454, I normally upload raw SFF file. Also, I split my total SFF file into each sample and link each sample to its ID on NCBI.

However, for illumina, I have a total file in FASTQ format. If I slit it into individual file, it will give me individual FASTA file (after de-multiplexier).

Should I upload individual FASTA file? Or total FASTQ file?

What format do you normally use for Illumina sequencing?

Thanks
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