Dear Members,
It is my pleasure to join this community.
I am a doctoral student in Japan.
I have a problem and I need help please. I was able to isolate to new unknown plasmids from halophilic bacteria and I was doing sequence of these plasmid by random DNA library construction. Now the sequence fragments of each plasmid doesnt show any overlap. I am nor sure what is the reason.
The purification was intensive to separate each plasmid but I would like to know if there is other possibilities for such a problem please.
I need your expert opinion as soon as possible.
Best Regards.
Omeya
It is my pleasure to join this community.
I am a doctoral student in Japan.
I have a problem and I need help please. I was able to isolate to new unknown plasmids from halophilic bacteria and I was doing sequence of these plasmid by random DNA library construction. Now the sequence fragments of each plasmid doesnt show any overlap. I am nor sure what is the reason.
The purification was intensive to separate each plasmid but I would like to know if there is other possibilities for such a problem please.
I need your expert opinion as soon as possible.
Best Regards.
Omeya
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