Tweet
I've seen 3'-5' coverage plots used to diagnose RNA degradation for RNA-Seq experiments. Before I begin writing my own I was just wondering if anyone knows of an existing script that can produce them (from a BAM and a GTF for instance)?
-
-
Nice, thanks!
I was also interested in some easy way to get what the geneBody_coverage.py module is plotting.Comment
-
Hi, have you tried ngs.plot? It is a very powerful and flexible tool to plot NGS samples. In addition to a global plot for the entire genome, you can create any combination of bam files and gene lists. You can plot RNA-seq or ChIP-seq which are implemented as two different modes.
As for your question, click this example .Last edited by asiangg; 06-19-2013, 09:42 AM.Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment