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  • STAR-Fusion with single-end reads?

    Hi,

    I have run STAR-Fusion successfully before, but never with single end reads. I have a new data set that I am trying to run it with and the error I get is:
    STAR-Fusion --genome_lib_dir /mnt/users/dgavino/genomefromfusion/GRCh38_gencode_v23_CTAT_lib/ \
    -J plasmaChimeric.out.junction \
    --output_dir star_fusion_outdir

    died with ret 65280 at /opt/STAR-Fusion/PerlLib/Pipeliner.pm line 79.
    Pipeliner::run(Pipeliner=HASH(0x204ecf8)) called at /usr/local/bin/STAR-Fusion line 259

    Anybody knows what that means? Did I do something wrong or is it bc of the single-end reads?

    Thanks,
    Danielle

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