Dear all,
I would like to create two complete bacterial genomes from two recently described bacteria. I have draft genomes for both. My plan is to create a second library using the 454. Is it likely that I would be able to close the genomes using this strategy?
For the draft assembly I used the Illumina MiSeq 250bp paired end reads and got x60 coverage. I then assembled the genomes using CLC genomics workbench and got these assemblies:
1. Contigs 92, N50 144,715, GC content 56%, size 5.2 Mb
2. Contigs 327, N50 38,957, GC content 53%, size 5.4 Mb
Thanks
I would like to create two complete bacterial genomes from two recently described bacteria. I have draft genomes for both. My plan is to create a second library using the 454. Is it likely that I would be able to close the genomes using this strategy?
For the draft assembly I used the Illumina MiSeq 250bp paired end reads and got x60 coverage. I then assembled the genomes using CLC genomics workbench and got these assemblies:
1. Contigs 92, N50 144,715, GC content 56%, size 5.2 Mb
2. Contigs 327, N50 38,957, GC content 53%, size 5.4 Mb
Thanks
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