Directional RNA sequencing protocols

[bluefin]

Hi,

Just wondering if anyone here had experience with the current directional RNA seq protocols and how well it performed.

thanks

[Nik]

Hi,

Yes, lots hjave been speaking about it. Have a read on other threads but it works. Two days but you will need sRNA and mRNA kits and buy some other reagents in. Bit costly so I wuld ask illumina when the universal kit will be released...

[das]

I used the protocol and it worked fine. Comparable data to standard protocol which I did in parallel for the same samples.
Let me know if you have specific questions.

DAS

[link1]

I'm gonna try both protocols next week and will let everyone know how it goes,