Dear all, title says it all:
We are looking into the best/most efficient/cost effective way of fragmenting/barcoding/sequencing a ~20kb target/fragment from ~200 independent sources. Final read depth needs to be ~1000x to enable variant calling.
Any ideas?
We were initially looking at NexteraXT, but this will come to over £7000 just for library prep materials for 200 samples.
Does anyone know of a homebrew option that enables multiplexing? Something with barcoded random primers perhaps?
Cheers! Matt
We are looking into the best/most efficient/cost effective way of fragmenting/barcoding/sequencing a ~20kb target/fragment from ~200 independent sources. Final read depth needs to be ~1000x to enable variant calling.
Any ideas?
We were initially looking at NexteraXT, but this will come to over £7000 just for library prep materials for 200 samples.
Does anyone know of a homebrew option that enables multiplexing? Something with barcoded random primers perhaps?
Cheers! Matt
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