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Old 05-20-2015, 10:36 AM   #1
Bioo Scientific
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Location: Austin, Tx

Join Date: Oct 2009
Posts: 99
Default mtDNA isolation and library prep

The NEXTflex™ mtDNA-Seq Kit includes optimized reagents for the isolation of mitochondrial (mtDNA) and construction of Illumina-compatible paired-end or single read libraries from genomic DNA. The NEXTflex mtDNA-Seq Kit enables selective enrichment of mitochondrial DNA from total genomic DNA samples by selectively digesting linear nuclear DNA (nDNA) while leaving circular mtDNA intact; enriching the mitochondrial genome 100 – 350 times. After enrichment libraries prepared using the NEXTflex mtDNA-Seq Kit produce a greater number of unique reads reducing the number of sequencing reads required per sample.

A whitepaper describing the mtDNA isolation and library prep technology can read here.

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heteroplasmy, mitochondrial dna, mtdna, mtdna-seq

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