Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • FASTA + FASTQ --> "FAST(q)" + FASTQ

    I need some help out here with an actually not that difficult task and useful to my field of research. Unfortunatley I cannot find a tool which makes my whishes come true -> maybe you can help me out !

    I have a fasta file and a fastq file.
    The fasta file had been corrected. (fake fastq can be used)
    The fastq data is raw uncorrected (with quality values of course).

    the task would be to map the multi fastq file to the multi fasta file. After mapping adding only the quality values to the corrected multi FASTA file. Thereby merging a corrected FASTA and a uncorrected FASTQ.

    FASTA + FASTQ --> "FAST(q)" + FASTQ

    That is the theory - can help some out with the practical approach ?

    Many thanks in advance
    Krid

  • #2
    You may think this is not difficult, but actually, it is. For example, what do you intend to do with reads that map to multiple locations? Or reads that map with indels? Or reads that map with lots of substitutions?

    Error-correction is very difficult and relies on assumptions of the error modes in the data needing correction. What kind of data do you have? Meaning library preparation, sequencing platform, etc.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    10 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    51 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    68 views
    0 likes
    Last Post seqadmin  
    Working...
    X