Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
Preprocessing needed for RNA-Seq data PFS Bioinformatics 10 03-06-2014 09:36 AM
how to order the reads of DNA methylation data from bisulfite sequencing Jim Michael Bioinformatics 7 10-24-2012 06:40 AM
RNA seq data preprocessing and cleaning figo1019 RNA Sequencing 0 07-17-2012 01:45 AM
Some queries on DNA methylation when processing the BS-seq data zeam Epigenetics 4 03-17-2011 08:40 AM

Thread Tools
Old 05-06-2014, 03:05 PM   #1
Location: Earth

Join Date: Jul 2011
Posts: 20
Default If I delete one sample do I have to redo DNA methylation data preprocessing

I have DNA methylation data for 532 samples. I did process .idat files for all samples together and got normalized beta values for all samples. One out of 532 samples has missing beta vlaues for 5500 probes. I decided to delete this sample.
Question: Do I have to redo the data preprocessing? I think converting image data (.idat) to signal strength wouldn't change, but normalization would change with different set of samples, even if there is only one sample difference.
HeidiLee is offline   Reply With Quote
Old 05-08-2014, 10:28 AM   #2
Rick Westerman
Location: Purdue University, Indiana, USA

Join Date: Jun 2008
Posts: 1,104

You didn't say what program you used. That might help people figure out the answer. Also how long does the pre-processing take? If a short time then, sure, re-process.
westerman is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 10:35 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO