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Old 07-07-2010, 02:38 PM   #1
niceday
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Location: cambridge

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Default RNA fragmentation

Does anyone know of any covaris settings to fragment RNA? I'm trying to avoid chemical fragmentation if possible.
Thanks.
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Old 07-15-2010, 08:42 AM   #2
Hamid
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Hi,

Please process your RNA samples in 120ul of TE, or buffer EB, using a Covaris MicroTube. Please use the settings of 10%dc/5i/200cpb/75 seconds to generate fragments of around 200 bases.

Thank you

hamid
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