Hi there,
I am somewhat new to RNA-Seq so my questions will be a bit naive!
I have already used the DEseq, DEGseq and edgeR for plotting and graphs of RNA seq data but I want to find the mutations in RNA seq data and this can be done using these tools (like Samtools for variants)
Thanks!
I am somewhat new to RNA-Seq so my questions will be a bit naive!
- I had run Bowtie for the alignment purposes and will be using Samtools for variations. Just need a clarification, that .bam files will be containing the Variation data aligned with the reference genome... Right?
- One more quick question, what are the reference files for any sample files (which have the extension of .gbk or .fasta). What's the purpose of those reference files? I have searched a lot for these files but I am unable to have a clear concept about these files....
I have already used the DEseq, DEGseq and edgeR for plotting and graphs of RNA seq data but I want to find the mutations in RNA seq data and this can be done using these tools (like Samtools for variants)
Thanks!