SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
ChIP-Seq: Single-tube linear DNA amplification (LinDA) for robust ChIP-seq. Newsbot! Literature Watch 19 02-29-2016 08:08 PM
NEXTflex ChIP-Seq Kit for Improved Genome Wide Analysis of DNA-Protein Interactions Bioo Scientific Vendor Forum 0 11-07-2011 09:25 AM
PubMed: Genome-wide distribution of DNA methylation at single-nucleotide resolution. Newsbot! Literature Watch 0 04-23-2011 12:14 AM
ChIP-Seq: ChIP-Seq Using High-Throughput DNA Sequencing for Genome-Wide Identificatio Newsbot! Literature Watch 0 10-16-2010 03:00 AM
PubMed: Genome-wide identification of in vivo protein-DNA binding sites from ChIP-Seq Newsbot! Literature Watch 0 08-08-2008 06:02 AM

Reply
 
Thread Tools
Old 01-28-2012, 03:00 AM   #1
Newsbot!
RSS Posting Maniac
 

Join Date: Feb 2008
Posts: 1,439
Default ChIP-Seq: Single-tube linear DNA amplification for genome-wide studies using a few th

Syndicated from PubMed RSS Feeds

Single-tube linear DNA amplification for genome-wide studies using a few thousand cells.

Nat Protoc. 2012;7(2):328-38

Authors: Shankaranarayanan P, Mendoza-Parra MA, van Gool W, Trindade LM, Gronemeyer H


Abstract
Linear amplification of DNA (LinDA) by T7 polymerase is a versatile and robust method for generating sufficient amounts of DNA for genome-wide studies with minute amounts of cells. LinDA can be coupled to a great number of global profiling technologies. Indeed, chromatin immunoprecipitation coupled to massive parallel sequencing (ChIP-seq) has been achieved for transcription factors and epigenetic modification of chromatin histones with 1,000 to 5,000 cells. LinDA largely simplifies reChIP-seq experiments to monitor co-binding at chromatin target sites. The single-tube design of LinDA is ideal for handling ultrasmall amounts of DNA (
Newsbot! is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 12:32 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO