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  • cufflinks with collapsed reads files

    Hi Everyone,

    We use cufflinks and tophat for our RNA-seq analysis. In our previous protocol, we use FASTX collapser to collapse reads with same sequence into one before doing alignments using bowtie/tophat. Does cufflinks consider such files when calculating FPKM?

    Thanks,
    Last edited by SongLi; 11-19-2010, 12:20 PM.

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