Illumina Hi-seq clean reads definition

[Marco0208]

Hello Guys

I am new to NGS technology and unfamiliar with the illumina hi-seq instruments.

I wish to obtain a quote from a genetic company and they use illumina hiseq instrument. However, their charge standards are in M clean reads/sample ! I am confused of the definition clean reads ? and what does it represents in base pairs ?

Please kindly help me with this matter !

Cheers

[kmcarr]

"Clean reads" is not a standard term for Illumina sequencing. I suspect that "clean reads" has a specific definition created by the sequencing company you are considering. You really should ask them to define the term for you. The best we can do on SeqAnswers is guess at what they might mean by that.

[Marco0208]

Hello Kmcarr

Thanks, so what kind of data does illumina hiseq 2000 generates ?

I just wish to use it for Micro RNA sequencing

[kmcarr]

Hello Kmcarr

Thanks, so what kind of data does illumina hiseq 2000 generates?

I don't understand what you mean by "what kind of data does illumina hiseq 2000 generates?" If you mean what is the output file format, Illumina sequence data is most commonly provided as compressed (gzip) FASTQ files.

I just wish to use it for Micro RNA sequencing

O.K. you can do that.

Again, you should really be discussing these details with your sequencing center.