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Old 07-14-2016, 02:24 PM   #1
jfostel
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Default Custom primers on a MiniSeq?

We sent a custom read 1 seq primer that works fine on our NextSeq and MiSeq to two groups with MiniSeqs, and the result is a total failure to find clusters for both. Control runs (Phi X and priming with standard seq primer) confirm the libraries and instruments are working fine, and Illumina TS says annealing temperatures are the same on NextSeq and MiniSeq.

What else could be going wrong here? Has anyone successfully used a custom primer on a MiniSeq?
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Old 07-20-2016, 11:36 AM   #2
jfostel
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Just an update - the Custom Primer Guide for MiniSeq contained an error. These seem to be the corrected positions:

Custom Read 1 primer: Position 15
Custom Read 2 primer: Position 14
Custom Index 1 primer: Position 13
Custom Index 2 primer: Position 13
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