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Old 10-09-2018, 01:05 PM   #1
ntremblay
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Location: Montreal, Quebec, Canada

Join Date: Dec 2009
Posts: 29
Question AAAAAA.. TTTTTTT.. heterodimers

Good evening all,

I have prepared (ds)cDNA from viral RNA extracted from plasma that was subsequently sequenced on a MiSEQ following standard library preparation.

When I got the reads back, I only have TTTTTTTTTTTTTT or AAAAAAAAA sequences for 99.9% of my reads. Read have good phred score and are of the expected length (100 bp).

Any ideas about why I might get such a result?

Thanks!

Nicolas
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Old 10-09-2018, 10:53 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,177
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Could you post full sequence of few reads (both reads if sequenced paired end).
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Old Today, 09:17 AM   #3
ntremblay
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Location: Montreal, Quebec, Canada

Join Date: Dec 2009
Posts: 29
Default

@m02846:68:000000000-c39d4:1:1101:14422:2079 1:n:0:1
tttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
+
>>afggggegggggggggggggggggggggggfggggggggggggggfffffffffffffffffffffffffffffffffffffffffffffffffffabffffffffffffffffffffffffffffffffffffffffff
@m02846:68:000000000-c39d4:1:1101:14422:2079 2:n:0:1
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
+
[email protected][email protected]@@@@@@@@@@@@@@[email protected]@@@@@@@@@@@@@@@@@@[email protected][email protected]<@@@@@@@@[email protected][email protected]@[email protected]@@@@@@@@@@[email protected]@@@@[email protected]@;;;=?;[email protected]@@[email protected]
@m02846:68:000000000-c39d4:1:1101:14218:2110 1:n:0:1
tttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
+
>>[email protected]@@/<[email protected]@cdgcbf?9bdab;dffffffbffffba=affffaffbbfbfffaafffaf;fffdffffffbd=ddf-9>[email protected][email protected]@bdf;[email protected];9=
M02846:68:000000000-C39D4:1:1101:14218:2110 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@[email protected]@@@@@@@@@@@@@@@@@@@@@[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@@@@@@;[email protected]>[email protected]@;@
@M02846:68:000000000-C39D4:1:1101:13886:2230 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>>AGGGGGGGGEEFGGGGFE/@[email protected][email protected]>DFFFAA;@[email protected]=FBFAABCF;BDDDC-B;-B;FD;>[email protected]@[email protected][email protected]
@M02846:68:000000000-C39D4:1:1101:13886:2230 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@@@@@@@@@@@@@@@@@@@@@[email protected]@@[email protected]@@@
@M02846:68:000000000-C39D4:1:1101:12194:2260 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>>AFGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGFFGFGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAAFFFFFA=DFFFA;FF;AFFFFFFFFFFFAFF;;ADAFFAFCFFFFAC;C
@M02846:68:000000000-C39D4:1:1101:12194:2260 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@@@@@@@@@@@@@@@
@M02846:68:000000000-C39D4:1:1101:12722:2310 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>>[email protected]/[email protected]@@[email protected]?BBBBB;@B?;[email protected][email protected]?B?B?B;>[email protected]>;-;BBB99B-B;[email protected];[email protected];=>@;[email protected]
@M02846:68:000000000-C39D4:1:1101:12722:2310 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@[email protected]@@@@@@@@@@@@@@@@@[email protected]@@@@@@@@@@[email protected][email protected]@@@@@@@@[email protected]@@@[email protected]@@@@@@@@@@@@@@@@@[email protected]@@@[email protected]@@@@@@[email protected]@@
@M02846:68:000000000-C39D4:1:1101:20711:2313 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>>AGGGGGGGGGGGGGGGGGGGCFGFGGGGCCGGGGGGGGGGGGGGGFAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFCAFFFFFFCFFFFFFFFFFFFFFFFFFAFFFFFAFFFFFF
@M02846:68:000000000-C39D4:1:1101:20711:2313 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@@
@M02846:68:000000000-C39D4:1:1101:17123:2486 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTGTTTTGGTGGGGTTTTTTGTGACTTCGACACTGTTT
+
>BBGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFDFA=D----.0000://.-<..--.-...--</00000....-:0000
@M02846:68:000000000-C39D4:1:1101:17123:2486 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@<[email protected]@@[email protected]@[email protected]@[email protected][email protected]@[email protected];[email protected]@;=->[email protected]?;[email protected]<=;[email protected]@-
@M02846:68:000000000-C39D4:1:1101:19948:2714 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAATTTTAATATTTTCGGGTGGGCTATACAACTGCCTTTTTCCTTCTTTTCCT
+
>>[email protected]@@GG<FFFFFFFFFFFFFFFFFFFFFFFDFFFFFAFFFFAF---9.////9///////;///--..---.;.///////;/////;/9/://;/////;
@M02846:68:000000000-C39D4:1:1101:19948:2714 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAACAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAACAAACC
+
[email protected]@@@@@@@@@@@[email protected]@@@@@@@@@[email protected][email protected]@@@[email protected][email protected]@[email protected]?<@@--9A--;@-9-;A-=---9-99;@---9-9-;-;////----------
@M02846:68:000000000-C39D4:1:1101:14946:3025 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@M02846:68:000000000-C39D4:1:1101:14946:3025 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
@M02846:68:000000000-C39D4:1:1101:15839:3063 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
3>>[email protected]@BBBB??B<BBBBBBB?;[email protected]?B<[email protected];BB?BB;B??BB?>=?;[email protected]@??BBBBB?--BB?-BBB?
@M02846:68:000000000-C39D4:1:1101:15839:3063 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@@@[email protected]@@@@@@@
@M02846:68:000000000-C39D4:1:1101:10519:3107 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>>AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;BFFFFFFFFAFAFFFF
@M02846:68:000000000-C39D4:1:1101:10519:3107 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@[email protected]@@@@@@[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@@@@[email protected]@@@@@@@@@@@@@@@@[email protected]@@@@@@[email protected][email protected]@@@@[email protected]@@@
@M02846:68:000000000-C39D4:1:1101:14081:3115 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>>AGGGGGGGEGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFAFFFFADFFFFFFFFFFFFFFFFFAFFFAFFFFFFFFFABFAFAFFFFFAF;DFFFFFFFAFAFFFAFFFAFFFFFFFFFAFFAFFAACFFACC
@M02846:68:000000000-C39D4:1:1101:14081:3115 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
@M02846:68:000000000-C39D4:1:1101:10255:3290 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>>[email protected]FFFFFFFFFFFFFFFFAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFAFFFACFFFFFFFFFAFFF
@M02846:68:000000000-C39D4:1:1101:10255:3290 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
@M02846:68:000000000-C39D4:1:1101:20826:3326 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@M02846:68:000000000-C39D4:1:1101:20826:3326 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
@M02846:68:000000000-C39D4:1:1101:9032:3354 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>>AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFFFFFFFFAFFFFFFFAFFFFFFFFFFFFFFFFCAFF
@M02846:68:000000000-C39D4:1:1101:9032:3354 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
@M02846:68:000000000-C39D4:1:1101:21178:3402 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
>>AFGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFAFFFFFFFFFFFFFFFFFFFFAAFFFFFFFFFFF;DACFFFFFFFFFFF-DFFFFAAFFAFFFFFFFFFBFF
@M02846:68:000000000-C39D4:1:1101:21178:3402 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@@@@@@@@@@@@@@
@M02846:68:000000000-C39D4:1:1101:18043:3408 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
3>AEGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFF-BBFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFCFAA;BFFFFFFFFFFFFFFFFFFFFFFAAB;@B--;--;;-;
@M02846:68:000000000-C39D4:1:1101:18043:3408 2:N:0:1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
[email protected]@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@[email protected]@@[email protected]@@@@@[email protected][email protected]@@@@@@@@@
@M02846:68:000000000-C39D4:1:1101:12925:3455 1:N:0:1
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTATACTTAATTTCTTTGAGTGTCTGAGTAATGTCTGACACAGCCACCTTATTATT
+
>>[email protected]@[email protected]@GBBBBBB?BBBBB??<[email protected]?BBBBB?BB?B;B=BB;B->-9/////////////////.;///9//;///////////9/...9....///;9/9
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