I know it's a discussed argument, but I'd like to have an opinion about my Bioanalyzer traces.
I extracted total bacterial RNA from food samples, then I used the Bacteria RiboZero kit to purify from rRNA. I don't know how my total RNA looked like before the RiboZero depletion, since I didn't run it on the BioAnalyzer, but after the rRNA depletion it looked like in the BA traces attached.
The sequencing core said I can't go ahead with the library preparation… But I saw on the RiboZero website that after the purification, I should have only a peak at 100-140nt, that is what I have. I can't understand why my traces are different from what epicenter write on the website.
Any comments about this? Does anyone has ever obtained libraries from this kind of mRNA?
I extracted total bacterial RNA from food samples, then I used the Bacteria RiboZero kit to purify from rRNA. I don't know how my total RNA looked like before the RiboZero depletion, since I didn't run it on the BioAnalyzer, but after the rRNA depletion it looked like in the BA traces attached.
The sequencing core said I can't go ahead with the library preparation… But I saw on the RiboZero website that after the purification, I should have only a peak at 100-140nt, that is what I have. I can't understand why my traces are different from what epicenter write on the website.
Any comments about this? Does anyone has ever obtained libraries from this kind of mRNA?
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