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12-16-2017, 06:47 PM
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#1 |
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Junior Member
Location: new york Join Date: Dec 2017
Posts: 4
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Getting rid of primer dimer for RNA-Seq libraries
Hey all, I just found several of my RNA-Seq libraries have primer dimers, I was wondering what is the ratio of my final product that is now diluted in resuspension buffer to AMPure beads to get rid of the primer dimers?
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12-16-2017, 08:32 PM
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#2 |
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Jafar Jabbari
Location: Melbourne Join Date: Jan 2013
Posts: 1,238
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You will need to do a second clean up with the same bead/DNA ratio used for clean up if you have been following a kit protocol. You will need to do second clean up on all libraries to avoid batch affect.
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12-16-2017, 09:10 PM
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#3 |
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Junior Member
Location: new york Join Date: Dec 2017
Posts: 4
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So I used trueseq, they recommend using 1:1 ratio, so can I proceed with 1:1
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12-16-2017, 11:29 PM
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#4 |
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Jafar Jabbari
Location: Melbourne Join Date: Jan 2013
Posts: 1,238
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That should be fine, just make sure that your pipette is accurate and use the same pipette for measuring library volume and bead.
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| Tags |
| ampure beads, primer dimer, rna-seq |
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