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Old 11-30-2020, 09:33 AM   #1
drops
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Location: Barcelona

Join Date: Nov 2011
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Default Nanopore library on top of an Illumina library

Hi all,
we don't have the original aliquote of an old sample, but we have an Illumina Nextera Flex library in the freezer.

I am aware that the ends are y-shaped, correct? Anyone of you have a fancy protocol to remodel/fill/modify those ends that I can do a Nanopore library on top?

Thx!
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Old 11-30-2020, 09:45 AM   #2
luc
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No remodeling needed.
All Nextera libraries are PCR amplified to be sequenceable. After the PCR amplification they are wonderfully double-stranded throughout and should be blunt-ended (if a high-fidelity polymerase was used).


BTW, Truseq-style libraries have Y-adapters; Nextera/transposase libraries work differently.
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