aligning the reads

kjaja · 09-27-2011, 06:32 AM

Hi,

I am planning to use CASAVA to align the reads to the reference genome using the fastq files. I was wondering if there are any quality control filtering steps that needs to be done before aligning the reads?

Thanks for your help

Kay

GenoMax · 09-27-2011, 06:50 AM

Kay,

If you are going to use the new version of CASAVA (v.1.8) you may need to remove the reads that would be normally filtered out before you do the alignments. See "dawe's" post on this page: http://seqanswers.com/forums/showthr...highlight=dawe

Illumina is supposed to come out with a update for CASAVA (http://seqanswers.com/forums/showthread.php?t=14316) that will do this automatically in October.

brojee · 07-07-2019, 07:45 AM

Hello there,

In the event that you are going to utilize the new form of CASAVA (v.1.8) you may need to expel the peruses that would be typically sifted through before you do the arrangements. See "dawe's" post on this page: http://seqanswers.com/gatherings/sho...highlight=dawe

Illumina should turn out with an update for CASAVA (http://seqanswers.com/discussions/sh...ad.php?t=14316) that will do this consequently in October.

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09-27-2011, 06:32 AM	#1
kjaja Member Location: NY Join Date: Aug 2011 Posts: 55	aligning the reads Hi, I am planning to use CASAVA to align the reads to the reference genome using the fastq files. I was wondering if there are any quality control filtering steps that needs to be done before aligning the reads? Thanks for your help Kay

07-07-2019, 07:45 AM	#3
brojee Member Location: Bhopal Join Date: Jul 2019 Posts: 19	Hello there, In the event that you are going to utilize the new form of CASAVA (v.1.8) you may need to expel the peruses that would be typically sifted through before you do the arrangements. See "dawe's" post on this page: http://seqanswers.com/gatherings/sho...highlight=dawe Illumina should turn out with an update for CASAVA (http://seqanswers.com/discussions/sh...ad.php?t=14316) that will do this consequently in October. __________________ Best SEO Company in Bhopal, Best Web Development Company in Bhopal

09-27-2011, 06:50 AM	#2
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 7,080	Kay, If you are going to use the new version of CASAVA (v.1.8) you may need to remove the reads that would be normally filtered out before you do the alignments. See "dawe's" post on this page: http://seqanswers.com/forums/showthr...highlight=dawe Illumina is supposed to come out with a update for CASAVA (http://seqanswers.com/forums/showthread.php?t=14316) that will do this automatically in October.