Questions about solexa quality score！ - Page 2

baohua100 · 02-20-2009, 06:09 AM

Now we get new solexa data:

@7_1wo1J99L221/1
GGAGTTGGGGTTCAGATGAAGNAGAGAAAGACCGTAGAAGTTCA
+
UUUUUUUUUUUUUUUQUUUUUEQUQUMMUUPUUUPMPMPPJPPP
@7_Xwo1J99L221/1
GTGTGAAGGTAACCGTAATGTNTGTTGACAAACGCCGTAACGAT
+
UUUQUORRURUUUUUUUUUUUEUUUUUQUUUNNEUPPPPPPPGP
@3_avo1J99L221/1
CAGGGATGGGATATAGTTTGCNTCGGTTTTAATAGTTGGGGGAG
+
UOUUSUUUUUUUUUUURUUUUEUUUUUUUUUUOUUPPPPPPPPP
@7_vuo1J99L221/1
GATGATGATGATGATGAGCTTNTCACTCGACGGTCACAGGCTTT
+
UUUUMUUUUUQUUUUUUUUUUEUUUUUUURUUUPUPPPPPPPMP

the most of char is U , how about this quality score mean ?

bioinfosm · 02-20-2009, 09:23 AM

Z is better and higher score than U..
More infor here - http://maq.sourceforge.net/fastq.shtml

BAJ · 02-23-2009, 02:17 AM

I found this table useful it contains both scores for solexa and phred...

http://www.genographia.org/portal/to..._download/file

It is in PDF fromat...

seq_GA · 05-21-2009, 12:36 AM

How to compare eland and maq quality score?
How to read maq quality score?
Thanks.

ABC@123 · 10-11-2020, 07:43 AM

These types of problems can be persisted if we don't try to fix these. Actually, there I was looking for help with the same problems and that would be good for me to get help with this.

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02-23-2009, 02:17 AM	#23
BAJ Member Location: Paris Join Date: Nov 2008 Posts: 15	conversion table for solexa and Phred I found this table useful it contains both scores for solexa and phred... http://www.genographia.org/portal/to..._download/file It is in PDF fromat...

10-11-2020, 07:43 AM	#25
ABC@123 Junior Member Location: canada Join Date: Oct 2020 Posts: 6	Re These types of problems can be persisted if we don't try to fix these. Actually, there I was looking for help with the same problems and that would be good for me to get help with this.

02-20-2009, 06:09 AM	#21
baohua100 Senior Member Location: Canada Join Date: Jun 2008 Posts: 103	Now we get new solexa data: @7_1wo1J99L221/1 GGAGTTGGGGTTCAGATGAAGNAGAGAAAGACCGTAGAAGTTCA + UUUUUUUUUUUUUUUQUUUUUEQUQUMMUUPUUUPMPMPPJPPP @7_Xwo1J99L221/1 GTGTGAAGGTAACCGTAATGTNTGTTGACAAACGCCGTAACGAT + UUUQUORRURUUUUUUUUUUUEUUUUUQUUUNNEUPPPPPPPGP @3_avo1J99L221/1 CAGGGATGGGATATAGTTTGCNTCGGTTTTAATAGTTGGGGGAG + UOUUSUUUUUUUUUUURUUUUEUUUUUUUUUUOUUPPPPPPPPP @7_vuo1J99L221/1 GATGATGATGATGATGAGCTTNTCACTCGACGGTCACAGGCTTT + UUUUMUUUUUQUUUUUUUUUUEUUUUUUURUUUPUPPPPPPPMP the most of char is U , how about this quality score mean ?

02-20-2009, 09:23 AM	#22
bioinfosm Senior Member Location: USA Join Date: Jan 2008 Posts: 482	Z is better and higher score than U.. More infor here - http://maq.sourceforge.net/fastq.shtml

05-21-2009, 12:36 AM	#24
seq_GA Senior Member Location: Asiana Join Date: Feb 2009 Posts: 124	How to compare eland and maq quality score? How to read maq quality score? Thanks.