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Old 08-04-2020, 09:40 AM   #1
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Question 16S & 18S Deep Sequencing Request(s)


We've done 16S/18S/ITS/amplicon sequencing for years, with good results - sometimes excellent (library dependent) - for both internal and external clients.

We're starting to see more and more requests for guaranteed extremely high read depth per sample - like in the neighbourhood of 50,000 to 100,000 reads per side per PE sample. What kind of Illumina instrument (other than a MiSeq) does this (amplicon) sequencing well and why are people becoming obsessed with ultra-high read depth?

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Old 08-04-2020, 10:47 PM   #2
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Hmm e-dna?
environmental DNA?
deep metagenomics?

I think Ion Torrent does a fairly good job of this ..
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16s, amplicon sequencing, illumina, miseq, read depth

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