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SOLiD v. 4 chemistry was touted to be able to reach 300,000 beads/panel which would produce just over 700 million beads/slide. But our record density was just over 550 million beads on a slide and required deposition of >1.4 billion beads. 2X overage.
Due to issues we attribute to extreme overages, we decided to dial back to a maximum of 1.5X overage of beads. This seems to land us in the 450 - 500 million bead range.
Is anyone getting higher densities that this? If so, any tips you want to offer us mortals?
Theoretically we should be able to pack somewhere north of 1.3 billion beads on a slide. (I am estimating, conservatively, at 1" x 3" for the slide with maybe 70% of its surface available after the 1 region gasket is applied. About 1.35 billion square microns.)
But even getting half that number is looking like a pipe dream.
This might be enrichment issues -- there might be lots of weak beads that don't get counted as "usable". I don't know.
We are still using manual methods, not EZ Bead.
--
Phillip
Due to issues we attribute to extreme overages, we decided to dial back to a maximum of 1.5X overage of beads. This seems to land us in the 450 - 500 million bead range.
Is anyone getting higher densities that this? If so, any tips you want to offer us mortals?
Theoretically we should be able to pack somewhere north of 1.3 billion beads on a slide. (I am estimating, conservatively, at 1" x 3" for the slide with maybe 70% of its surface available after the 1 region gasket is applied. About 1.35 billion square microns.)
But even getting half that number is looking like a pipe dream.
This might be enrichment issues -- there might be lots of weak beads that don't get counted as "usable". I don't know.
We are still using manual methods, not EZ Bead.
--
Phillip