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Old 07-10-2017, 05:39 AM   #1
balaena
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Location: Germany

Join Date: Jan 2015
Posts: 29
Default NCBI genome submission, alternative start codons

Hi,

I wonder if anyone knows how one can tell NCBI submission tools to use alternative start codons for an annotated prokaryote genome.

I used GAG to convert the GFF containing the gene predictions into TBL for tbl2asn. I also let it fix the start and stop codons. But a certain amount of genes (10%) apparently use alternative start codons and GAG does not recognize that. tbl2asn then gives a "fatal" error because prokaryote genes are not allowed without start or stop.

Any idea how to fix that?
Thanks!
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