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Old 05-18-2015, 08:25 PM   #1
angel-sakura
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Location: Aus

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Smile how to check the primers specificity in a local computer

Could someone please tell me how to check the specificity of primers in a local computer? I know there are lots of online tools available, i.e. primer-blast. However, the database of my research model is not updated in ncbi or any other database. Now I have a better database available in my local computer and was trying to set up a local primer-blast system. But I cannot find the download link of primer-blast or any replacement softwares to check the specificity of my primers with my local database. Could someone please shed light on this?

Thanks a lot.
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Old 05-19-2015, 02:14 AM   #2
GenoMax
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If you are looking to confirm specificity of the primers you have already designed then you can use blat locally (https://genome.ucsc.edu/FAQ/FAQblat.html). You will need a unix machine. With blat a multi-fasta file containing your genome sequence can serve as the "database".
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Old 05-19-2015, 03:58 AM   #3
angel-sakura
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Thank you very much!!
That's is what I want

Cheers
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