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Old 04-21-2011, 07:47 AM   #1
PGT_Bordeaux
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Location: Bordeaux France

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Default MiSeq

Hello everybody,

I'm new, I work in facility of Bordeaux (France).
I have a GAIIx since November 2010, on this machine all is ok.

I saw the new sequencer illumina, the MySeq,
What some people are using it ?

manip time for make library ? on paper, time preparation is very fast ...
Multiplexing on 96 samples ??
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Old 04-24-2011, 02:07 PM   #2
krobison
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MiSeq will not be available in quantity until the end of 2011; none have been officially sold yet (anyone out there alpha testing one?)
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Old 07-25-2011, 12:26 PM   #3
DNA Sorcerer
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I though that library prep is done the same way as for GAIIx.
The main differences are in throughput. Myseq has only one "lane" and gives about 1GB when doing 150bp PE in abut 27Hs. Of course myseq is way smaller and all the modules are built in.
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Old 07-26-2011, 07:31 PM   #4
ShaunMahony
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Keith -- did you ever find anyone who is testing a MiSeq? Especially in the Boston area? I'm interested in talking to someone with experience on the platform.
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Old 07-27-2011, 07:00 AM   #5
krobison
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Shaun: No, I haven't seen one in the flesh yet. I'd also love to hear first hand knowledge of it.

There is an Illumina user's meeting in Cambridge MA this September 8th; hard to imagine that MiSeq won't figure prominently.
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Old 07-27-2011, 07:05 AM   #6
ShaunMahony
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Thanks for the heads-up about the Illumina meeting!
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Old 07-29-2011, 07:36 PM   #7
penpen
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Quote:
Originally Posted by krobison View Post
MiSeq will not be available in quantity until the end of 2011; none have been officially sold yet (anyone out there alpha testing one?)
hi krobison, do u know the firm shipping date of miseq. illumina rep promise us to give one at the end of june, but it seems delayed...
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