SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
BS-Seq from a single cell? eslondon Epigenetics 1 07-26-2011 11:43 PM
Library for single cell RNA seq??? zianeffy Sample Prep / Library Generation 2 07-05-2011 12:46 PM
RNA-Seq: Transcript amplification from single bacterium for transcriptome analysis. Newsbot! Literature Watch 0 05-04-2011 02:50 AM
mRNA-Sequencing Whole Transcriptome Analysis of a Single Cell on the SOLiD System GeeSharpMinor Literature Watch 0 12-02-2009 03:44 PM
PubMed: High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Newsbot! Literature Watch 0 04-16-2008 07:51 AM

Reply
 
Thread Tools
Old 04-26-2009, 11:12 PM   #1
benimmyeo
Junior Member
 
Location: Singapore

Join Date: Dec 2008
Posts: 3
Default mRNA-Seq whole-transcriptome analysis of a single cell.

ARTICLE ABSTRACT
Nature Methods
Published online: 6 April 2009 | Corrected online: 19 April 2009 | doi:10.1038/nmeth.1315

mRNA-Seq whole-transcriptome analysis of a single cell

Fuchou Tang1,3, Catalin Barbacioru2,3, Yangzhou Wang2, Ellen Nordman2, Clarence Lee2, Nanlan Xu2, Xiaohui Wang2, John Bodeau2, Brian B Tuch2, Asim Siddiqui2, Kaiqin Lao2 & M Azim Surani1

Abstract
Next-generation sequencing technology is a powerful tool for transcriptome analysis. However, under certain conditions, only a small amount of material is available, which requires more sensitive techniques that can preferably be used at the single-cell level. Here we describe a single-cell digital gene expression profiling assay. Using our mRNA-Seq assay with only a single mouse blastomere, we detected the expression of 75% (5,270) more genes than microarray techniques and identified 1,753 previously unknown splice junctions called by at least 5 reads. Moreover, 819% of the genes with multiple known transcript isoforms expressed at least two isoforms in the same blastomere or oocyte, which unambiguously demonstrated the complexity of the transcript variants at whole-genome scale in individual cells. Finally, for Dicer1-/- and Ago2-/- (Eif2c2-/-) oocytes, we found that 1,696 and 1,553 genes, respectively, were abnormally upregulated compared to wild-type controls, with 619 genes in common.
benimmyeo is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 04:23 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO