SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
Gel-size selection in NimbleGen exon capture mitchelS Illumina/Solexa 3 10-26-2012 08:50 AM
Post PCR Enrichment Gel Selection for ChIP-Seq Daytwa Sample Prep / Library Generation 7 05-10-2012 07:06 AM
weird gel-size selection of 454 library havana Sample Prep / Library Generation 0 03-22-2012 12:29 AM
PE (in gel) Size-Selection puggie Illumina/Solexa 3 11-28-2011 04:24 AM
Nextera Problem: Odd fragment size after gel selection Kitty Sample Prep / Library Generation 3 09-15-2011 03:54 AM

Reply
 
Thread Tools
Old 10-23-2012, 06:57 AM   #1
FredBeaudoin
Junior Member
 
Location: Sherbrooke, Qc, Canada

Join Date: Sep 2012
Posts: 4
Default Gel used for size selection

Hi there,

We are doing our home made protocol for RNAseq and smallRNAseq. After budgetting, i found out that the gels used for gel size selection are quite expensive (15$ to 20$) for pre-cast gel. I look for hi resolution agarose that could be not so expensive? any company recommandation?
FredBeaudoin is offline   Reply With Quote
Old 10-31-2012, 12:23 AM   #2
Simone78
Senior Member
 
Location: Basel (Switzerland)

Join Date: Oct 2010
Posts: 207
Default

Hi,
if your product is <1000 bp you can try NuSieve® GTG® Agarose (Lonza). In my hands it gave a much sharper band (and yield) than other low-melting agarose.
Good luck!
Simone78 is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 05:35 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO