Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Qubit library quantitation variations Smriti Sample Prep / Library Generation 5 11-02-2013 09:57 AM
qubit/picogreen seqgirl123 Sample Prep / Library Generation 4 11-02-2013 07:06 AM
Can you use PicoGreen in a Qubit assay? crader General 2 12-05-2012 10:35 AM
Quality Control Library using QUBIT... help me! soleulloa Ion Torrent 1 09-01-2011 06:18 AM
Recover RNA from Qubit measurement RickC7 Sample Prep / Library Generation 0 06-03-2011 12:52 PM

Thread Tools
Old 09-13-2010, 03:59 AM   #1
Location: USA

Join Date: Jul 2010
Posts: 58
Default QuBit fluorimeter vs. Epoch?

Dear all,

I measured DNA concentration with two way, QuBit and Epoch.
For NimbleGen exome capture in Illumina sequencing, DNA is needed 30ng/ul.

But DNA concentration measured by Epoch (900ng/ul) was way higher than DNA measured by Qubit(700 ng/ul). I used DNA BR kit for QuBit.

Is Qubit more reliable? I assumed that epoch cannot detect low concentration well.

Last edited by sehrrot; 09-13-2010 at 05:28 PM.
sehrrot is offline   Reply With Quote
Old 09-16-2010, 04:55 AM   #2
Location: Eesti

Join Date: Jan 2009
Posts: 37

I am afraid none of them is reliable. When possible use the most conservative value.
v_kisand is offline   Reply With Quote
Old 09-16-2010, 07:22 AM   #3
Location: cambridge

Join Date: Apr 2010
Posts: 68

we use Qubit. We find it gives values slightly higher than the bioanalyser but we base our conc's and experiments on the Qubit readings and haven't had any problems. We also use Qubit and bioanalyser after fragmentation...basically whenever the protocols say bioanalyser we also do Qubit.
niceday is offline   Reply With Quote
Old 09-16-2010, 11:09 AM   #4
Senior Member
Location: Amsterdam

Join Date: Nov 2009
Posts: 112

For 30 ng/ul I would use the HS kit on the Qubit.
Zaag is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 12:39 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO