SEQanswers

Go Back   SEQanswers > Bioinformatics > Bioinformatics



Similar Threads
Thread Thread Starter Forum Replies Last Post
how to use mira to assemble the fastq generated by 454 sequencing dingkai0564 Bioinformatics 6 05-26-2013 01:12 PM
ELAND to FASTQ gokhulkrishnakilaru Bioinformatics 0 02-13-2012 10:08 AM
How to do CASAVA alignment by using fastq files weasteam Bioinformatics 2 01-03-2012 11:18 AM
FindPeaks SeparateReads.jar error using GERALD/ELAND-generated files AdamB Bioinformatics 7 03-31-2011 07:09 AM
What do i do with the generated files? BRCA-DIAGNOSTIC andrehorta Bioinformatics 0 01-13-2011 02:01 AM

Reply
 
Thread Tools
Old 11-18-2010, 02:20 PM   #1
casava
Junior Member
 
Location: CA

Join Date: Nov 2010
Posts: 1
Default fastq files generated by Casava-Eland

Hi,
I know the eland export files generated from Eland in the Casava software contain recalibrated quality scores. Does anyone know if the fastq files generated by Casava also contain the recalibrated quality score or the original quality scores?
Thanks,
Casava
casava is offline   Reply With Quote
Old 11-19-2010, 04:56 AM   #2
kmcarr
Senior Member
 
Location: USA, Midwest

Join Date: May 2008
Posts: 1,178
Default

It is my understanding that the Illumina pipeline no longer performs quality score re-calibration, and Illumina generally recommends against it.
kmcarr is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:35 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO