we used the adapter with a 3-bases tag in the 3' terminator.
The PCR primers are the universe primers from illumina, 16 cycles
the PCR products are tested by Agilent 2100, the results are below attachment.
as you see, noncovalent concatamerization of fragments (“bird-nesting”) was observed. so, how to optimize the conditions to reduce the bird-nesting.
The PCR primers are the universe primers from illumina, 16 cycles
the PCR products are tested by Agilent 2100, the results are below attachment.
as you see, noncovalent concatamerization of fragments (“bird-nesting”) was observed. so, how to optimize the conditions to reduce the bird-nesting.
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