Any help would be much appreciated, I am trying to get tBlastx to work but not having any luck (error message and query in attached image).
I have a single amino acid sequence to "BLASTx" to a large list of nt sequences. If I run the same set under tBlastn, it works perfectly, so I should be able to use the exact same query and subject for tBlastx, right?
When I try, get get an error that a protein was used for nt, but why does it work for tblastn?
Thanks everyone![Smile](https://www.seqanswers.com/core/images/smilies/smile.png)
I have a single amino acid sequence to "BLASTx" to a large list of nt sequences. If I run the same set under tBlastn, it works perfectly, so I should be able to use the exact same query and subject for tBlastx, right?
When I try, get get an error that a protein was used for nt, but why does it work for tblastn?
Thanks everyone
![Smile](https://www.seqanswers.com/core/images/smilies/smile.png)
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