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  • #1

    General coverage questions for NGS

    How many flow cells do I need for sequencing a human genome with 30X converage (paired-end)?
    Does the number of reads related with the number of lanes to use in a flow cell?

    If I want to sequence 8 microbial genomes de novo, each of the 8 genomes is 10 Mbases in size.
    How many 100bp paired-end reads will be needed to achieve 50X coverage for each of the genomes? Will you suggest multiplexed sequencing?
    Tags: coverage calculation, illumina, paired end reads
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