Hi all,
I've used a MiSeq 500 cycles v2 and 10 pM of my libraries pool + phiX 10%.
Everything was ok but at cycle 491 the run was stopped (I don't know why).
I was looking for some paramethers and that is the information:
1249 mm/k2
R1: 88.16% cluster PF, 85.13% >Q30
R2: 88.16% cluster PF, 46.22% >Q30
Why the quality was so low in R2 sequences? It never ocurrs before ☹️
Thank you!!!
Soledad
I've used a MiSeq 500 cycles v2 and 10 pM of my libraries pool + phiX 10%.
Everything was ok but at cycle 491 the run was stopped (I don't know why).
I was looking for some paramethers and that is the information:
1249 mm/k2
R1: 88.16% cluster PF, 85.13% >Q30
R2: 88.16% cluster PF, 46.22% >Q30
Why the quality was so low in R2 sequences? It never ocurrs before ☹️
Thank you!!!
Soledad
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