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I have been using CLC Genomics for RNA-seq analyses. I am using the gaussian-based tests in CLC Genomics to detect differentially expressed genes but I am interested in using Bioconductor/R packages, such as DESeq. Can I use the exported bam/sam files produced from CLC's RNA-seq mapping tool in DESeq? Or do I need to use bam/sam files from another mapper such as TopHat? Additionally, can I use the bam/sam files from CLC as input into the HTSeq-count program? Thank in advance for you help.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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