PubMed: Massively parallel sequencing, a new method for detecting adventitious agents

Newsbot! · 03-27-2010, 02:05 AM

Syndicated from PubMed RSS Feeds

Massively parallel sequencing, a new method for detecting adventitious agents.

Biologicals. 2010 Mar 23;

Authors: Onions D, Kolman J

There has been an upsurge of interest in developing new veterinary and human vaccines and, in turn, this has involved the development of new mammalian and insect cell substrates. Excluding adventitious agents from these cells can be problematic, particularly for cells derived from species with limited virological investigation. Massively parallel sequencing is a powerful new method for the identification of viruses and other adventitious agents, without prior knowledge of the nature of the agent. We have developed methods using random priming to detect viruses in the supernatants from cell substrates or in virus seed stocks. Using these methods we have recently discovered a new parvovirus in bovine serum. When applied to sequencing the transcriptome, massively parallel sequencing can reveal latent or silent infections. Enormous amounts of data are developed in this process usually between 100 and 400Mbp. Consequently, sophisticated bioinformatic algorithms are required to analyse and verify virus targets.

PMID: 20338780 [PubMed - as supplied by publisher]

More...

CodyFuller · 02-05-2018, 05:05 AM

The rule of the good and wonderful stints has been done for the success of the individuals. The strictness of the thebestessayservice.com has been formatted for the right and wonderful elements for the humans in life.

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
PubMed: Massively parallel sequencing for the detection of adventitious viruses.	Newsbot!	Literature Watch	0	02-02-2012 11:20 PM
RNA-Seq: The sensitivity of massively parallel sequencing for detecting candidate inf	Newsbot!	Literature Watch	0	05-24-2011 02:00 AM
PubMed: Massively parallel sequencing and rare disease.	Newsbot!	Literature Watch	0	09-18-2010 10:51 AM
PubMed: A method for studying protistan diversity using massively parallel sequencing	Newsbot!	Literature Watch	0	07-28-2009 05:00 AM
PubMed: Gene expression profiling by massively parallel sequencing	Newsbot!	Literature Watch	0	02-21-2008 02:06 PM

03-27-2010, 02:05 AM	#1
Newsbot! RSS Posting Maniac Join Date: Feb 2008 Posts: 1,443	PubMed: Massively parallel sequencing, a new method for detecting adventitious agents Syndicated from PubMed RSS Feeds Massively parallel sequencing, a new method for detecting adventitious agents. Biologicals. 2010 Mar 23; Authors: Onions D, Kolman J There has been an upsurge of interest in developing new veterinary and human vaccines and, in turn, this has involved the development of new mammalian and insect cell substrates. Excluding adventitious agents from these cells can be problematic, particularly for cells derived from species with limited virological investigation. Massively parallel sequencing is a powerful new method for the identification of viruses and other adventitious agents, without prior knowledge of the nature of the agent. We have developed methods using random priming to detect viruses in the supernatants from cell substrates or in virus seed stocks. Using these methods we have recently discovered a new parvovirus in bovine serum. When applied to sequencing the transcriptome, massively parallel sequencing can reveal latent or silent infections. Enormous amounts of data are developed in this process usually between 100 and 400Mbp. Consequently, sophisticated bioinformatic algorithms are required to analyse and verify virus targets. PMID: 20338780 [PubMed - as supplied by publisher] More...

02-05-2018, 05:05 AM	#2
CodyFuller Junior Member Location: USA Join Date: Feb 2018 Posts: 1	The rule of the good and wonderful stints has been done for the success of the individuals. The strictness of the thebestessayservice.com has been formatted for the right and wonderful elements for the humans in life.